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Qpt For Powerpoint Full 31

  • faron1991
  • Aug 17, 2023
  • 2 min read


Protein sequences of Arabidopsis thaliana ASO, QS, QPT, NMNAT, NS (listed in Table 1), human NAMPT (NP_005737), yeast SIR2 (NP_010242), and human SIRT4 (NP_036372) were used in TBLASTN against JGI (Joint Genome Institute) Chlamydomonas reinhardtii genome version 4.0 (JGI v4.0, -psf.org/Chlre4/Chlre4.home.html) with expected E-values less than or equal to 1E-5 (1E-3 for SIR2 and SIRT4). The resultant genes were checked for EST coverage. Genes without full-length EST coverage, QS, NMNAT, NS, NAMPT, SRT1, and SRT2, were subjected to exon-intron predictions using GreenGenie 2 ( -bin/greengenie2/greenGenie2) [33]. The predicted coding regions were used as guidelines in primer design for RT-PCR to amplify the actual coding regions of these genes.




Qpt For Powerpoint Full 31




Chlamydomonas BAC DNA was prepared using Qiagen Plasmid Midiprep kit. To prepare the pNIC15a plasmid, the BAC (32L22) DNA was digested with XmaI and a 6.1 kb fragment was isolated and cloned into a pBlueScript II SK vector (Stratagene, La Jolla, CA). This fragment contains a 1 kb upstream sequence, the full-length NIC15 gene, and a 2.5 kb downstream sequence, which is predicted to be part of an unknown zinc finger protein (protein id 150664). To prepare the pNIC1-56 plasmid, a 7.1 kb KpnI fragment from the BAC (10M24) DNA was cloned into a pBlueScript II SK vector. The plasmid contains a 0.7 kb upstream sequence, the full-length NIC1 gene, and a 4.7 kb downstream sequence, which is predicted to contain an unknown protein that has a HAD-superfamily hydrolase domain.


I'm trying to figure out what PP skills I need to develop now before going to b-school. I plan on applying to b-school in the fall and gun for consulting jobs but want to start practicing now before school since I am not a consultant. I heard advanced PP skills are needed similar to Excel skills for an IB associate. How advanced do I need to be to successfully complete tasks as an associate at MBB/2nd tier consulting firms? Are there any good tutorials out there or can I just learn on the job? Thanks. 2ff7e9595c


 
 
 

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